1、method
Using multiple fluorescent PCR technology, design specific probes and primers for detection of the ORF1ab and N gene regions of the new coronavirus
2、Performance analysis
(1) Good accuracy, testing national reference products and enterprise internal reference products, the compliance rate is 100%
(2) Precision, within-run precision variation coefficient CV≤5%
(3) The detection limit, the minimum detection concentration of the sample can be as low as 500 copies/mL, further reducing the risk of sample missed detection.
(4) Amplification efficiency, reagent amplification efficiency is between 0.95~1.1, R2>0.99, with good amplification ability.
4、Competitive product comparison
Weak positive sample results comparison
Figure 1 Three-channel amplification results of competing products
Figure 2 Three-channel amplification results of our company's products
